The present study investigated the anti-inflammatory effect of Jakyakgamcho-tang in lipopolysaccharide-exposed rats. The plasma concentration of IL-$1eta$, IL-6 and TNF-$alpha$ peaked at 5hrs after LPS injection, and these values of the 300 mg/kg Jakyakgamcho-tang group were lower than those of the control group. The plasma concentration of IL-10 peaked at 5hrs after LPS injection and the values of the Jakyakgamcho-tang groups were higher than those of the control group. In the liver cytokines measurement was done at 5hrs after LPS injection. The concentrations of liver IL-$1eta$ and IL-6 in the 300 mg/kg Jakyakgamcho-tang were lower than that of the control group. The concentrations of liver TNF-$alpha$, and IL-10 showed no significant differences among all the treatment groups. These results indicate that the Jakyakgamcho-tang has anti-inflammatory activities.