Recently H1N1(swine flu) and SARS has been infected widely in the world; we have to care about germs and virus in indoor air environment. The air sterilization system investigated in this study allows occupants to turn on/off and to control the incoming air speed and direction. To predict the performance of air sterilization system without real experiment, a simulation is considered to compare and analyze the performance of the air sterilization systems in a typical office space. Multiple system parameters including volume flow rate and velocity of supply air were varied and investigated during the simulation. The investigation result shows that difference (between simulation and experiment) was about 3.5% in case of minimum air flow rate and about 0.2% in case of maximum air flow rate. The results indicate that multi-zone simulation technique can be used to predict the performance of a sterilization system in personalized office.