Bumblebees are widely used to pollinate various crops, especially tomato, in greenhouses and fields. An artificial hibernation is essential for year-round rearing of the bumblebee, which passes through one generation per year. Here, we investigated whether a chilling temperature and humidity affect artificial hibernation of the bumblebee queen Bombus terrestris. In chilling temperature regimes of $0^{circ}C$, $2.5^{circ}C$, $5^{circ}C$, $7.5^{circ}C$ or $12.5^{circ}C$ under constant humidity >70%, the queens stored at $2.5^{circ}C$ exhibited the highest rate of survival, which was 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months, and 24.0% at 6 months. Rates of survival decreased at the following temperatures: $0^{circ}C$, $5^{circ}C$, $7.5^{circ}C$ and $12.5^{circ}C$. Colony developmental characteristics after diapause were 1.2- to 1.5-fold higher than those of queens stored at $5^{circ}C$. In terms of chilling humidity, the queens hibernated at 70% under $2.5^{circ}C$ exhibited the highest rate of survival, which was $93.3{pm}3.4%$ at one month, $83.3{pm}0.0%$ at two months, $76.7{pm}0.0%$ at 3 months and $36.7{pm}12.1%$ at 5 months. The rates of oviposition, colony foundation and progeny-queen production of queens hibernated at 70% were 80.8%, 30.8% and 30.8%, respectively. These values correspond to 1.7- to 3.3-fold increases in comparison to queens stored at 50% humidity. Therefore, $2.5^{circ}C$ and 70% R.H. were the favorable chilling temperature and humidity conditions for diapause break of B. terrestris queens.