본 연구는 지골피, 동충하초, 가시오가피의 복합추출물을 고포도당 조건(4.5 g/L)에서 배양한 HepG2 세포를 이용하여 당대사 관련 주요 효소인 glucokinase, acetyl CoA carboxylase의 활성을 RT-PCR과 western blotting 방법을 이용하여 측정하였다. 복합추출물을 HepG2 세포에 24시간 처리한 후 GK mRNA와 protein의 발현 양을 측정한 결과 각각 $168{pm}0.04%$, $182.4{pm}0.03%$로 증가하였으며, ACC 효소의 활성은 고포도당으로 배양하였을 때 ACC mRNA 및 protein 발현 양은 각각 $127.3{pm}0.02%$, $126.7{pm}0.24%$로 증가 하였다. 결론적으로 지골피, 동충하초, 가시오가피의 복합추출물이 GK 및 ACC mRNA 발현을 증가시킴으로써 항당뇨 효과를 나타냄을 관찰하였다.
This study investigated the anti-diabetic effects of the mixed water extract (JDG 100) composed of Lycii Cortex, Acanthopanax senticosus and Cordyceps militaris on glucose-regulating key enzymes such as glucokinase (GK), acetyl-CoA carboxylase (ACC). In the current study, HepG2 cells were exposed to pathological condition such as hyperglycemic condition (4.5 g glucose/L) with JDG 100 and then experiments such as RT-PCR and Western blotting were carried out. JDG 100 treated cells increased to $168{pm}0.04%$ and $182.4{pm}0.03%$ in GK mRNA and protein expressions, respectively, compared to control. Treatment of the JDG 100 up-regulated ACC mRNA ($127.3{pm}0.02%$) and protein ($126.7{pm}0.24%$) of HepG2 cells in the high glucose media. These observations suggest that JDG 100 mixed water extract may have a potential as an anti-diabetic agent in type 2 diabetes mellitus.
