An iris image pattern positioning with preserved edge detector, ring zone and clock zone, frequency distribution and overlay matching is presented in this paper. Edge detector is required to be powerful and detail. That is proposed by overlaying Canny with LOG (CLOG). The two reference patterns are made from allocating each gray level on the clock zone and ring zone respectively. The normalized target image is overlaid with the clock zone reference pattern and the ring zone pattern to extract overlapped number, and make a matched frequency distribution to look through a symptom and position of human organ and tissue. The iterating experiments result in the ring and clock zone positioning evaluation.