In this paper, we propose the localization method of mobile robot using SURF(Speeded-Up Robust Features) and Particle filter. The proposed method is as follows: First, we seek the Landmark from the obtained image using SURF in order to find the first rigorous position of mobile robot. Second, we obtain the distance from obstacles using ultrasonic sensors in order to create the relative position of mobile robot. And then, we estimate the localization of mobile robot using Particle filter about movement of mobile robot. Finally, we show the feasibility of the proposed method through some experiments.