This study was conducted to investigate whether Mulligan manual therapy and Physical therapy have effectiveness on the pain and muscle assessment questionnaire in female elders with osteoarthritis of the knee. Thirty subjects were participated in this study. And they were all randomly divided into Mulligan manual therapy and Physical therapy group. To evaluate the effects of Mulligan manual therapy and Physical therapy, subjects were evaluated by using visual analogue scale and muscle assessment questionnaire. The assessment parameters were evaluated before, after 2 weeks, and after 4 weeks treatments. And we received a consent form from Mulligan manual therapy subjects. The results of repeated measures analysis of variance showed that pain, strength, endurance, coordination/balance were significantly improved after than before therapy in Mulligan manual therapy group. So we conclude that Mulligan manual therapy has effectiveness on the pain and muscle assessment questionnaire in female elders with osteoarthritis of the knee.