The artificial upwelling of deep seawater increases primary production. This study conducted a lab-scale experiment to investigate the growth of phytoplankton with the mixing ratio of deep and surface seawater. The chlorophyll content in the sample of pure deep seawater was highest, regardless of the phytoplankton groups. Nutrients contained in the deep seawater positively influenced the growth of phytoplankton. The optimum mixture to apply in an artificial upwelling system was a 1:1 ratio of deep and surface seawater. An experiment considering other environmental conditions, such as luminance and specific gravity, should be performed.