This retrospective study was performed in 40 cases of canine crystalluria and urinary tract symptoms. Breed, age, sex, diet history and common clinical signs were investigated for patient information. CBC, serum chemistry, urinalysis, urine culture, urine sediment and urinary stone analysis were undertaken for clinicopathological diagnosis. Crystalluria and urinary tract symptoms occurred from 6 months till 14 years in this clinical investigation and the average age was 4.68 years. It had taken an average 7.4 weeks to correct crystalluria and urinary tract symptoms treated by supportive therapy and dietary management. In conclusion, regular clinical pathologic examination, treatment and diet management should be needed to prevent the formation of urolithiasis and urinary tract diseases in dogs with crystalluria and urinary tract symptoms.