For testing large-capacity pumps, the accurate flow rate measurement is needed in the test loop. As a measuring method of flow rate, venturi tube is recommended due to its low pressure loss. However, upstream disturbance of loop component such as a valve has an effect upon the accuracy of flow rate measurement. For controlling flow rate in case of high flow rate and large-scale piping system, a butterfly-type valve is generally used due to its compactness. However, a butterfly valve disturbs downstream flow by generating turbulence, cavities, or abrupt pressure change. In this study, the effect of downstream disturbance of butterfly valve on the flow rate measurement using a venturi tube is investigated. Test loop consists of circulation pump, reservoir, butterfly valve, venturi tube, and reference flow meter. The test is conducted with regard to a different valve opening angle of butterfly valve. According to the valve opening angle, the uncertainty of flow rate measurement is investigated.