The antifungal effects and action mechanisms of styraxjaponoside A and B were investigated. Devoid of hemolytic effect, the compounds had significant effect against several human pathogenic fungal strains, with energy-independent manners. To understand the action mechanisms of the compounds, the flow cytometric analysis plotting the forward scatter and the side scatter, $DiBAC_4$(3) staining and DPH fluorescence analysis were conducted. The results indicated that the actions of the compounds were dependent upon the membrane-active mechanisms. The present study suggests that styraxjaponoside A and B exert their antimicrobial effects via membrane-disruptive mechanisms.