This paper presents the Application of Response Surface Method for Optimal Transfer Conditions of MLCC Alignment System. his paper is composed of two parts: (1) Testing performance verification of MLCC alignment system, compared with manual operation; (2) Applying response surface method to figuring out the optimal transfer conditions of MLCC transfer system. Based on the successfully developed MLCC alignment system, the optimal transfer conditions have been explored by using RSM. The simulations using $ADAMS^{(R)}$ has been performed according to the cube model of CCD. By using $MiniTAB^{(R)}$, we have established the model of response surface based on the simulation results. The optimal conditions resulted from the response optimization tool of $MiniTAB^{(R)}$ has been verified by being assigned to the prototype of MLCC alignment system.