This study was conducted to evaluate the differences in symptoms of work-related musculoskeletal disorders (WMSDs) and health-related quality of life (HRQoL) between vinylhouse farmers and non-vinylhouse farmers. The study included 118 residents who lived in a rural community. All subjects were assigned to the vinylhouse farmers group (N=58) and the non-vinylhouse farmers group (N=60) according to main agricultural work type. All respondents were interviewed by means of a structured questionnaire. WMSDs symptoms were measured by a self- assessed questionnaire on symptoms of musculoskeletal disorders, and HRQoL was measured by SF-36. Vinylhouse farmers had significant more symptoms of WMSDs in each regional parts of musculoskeletal system, and had significant poor total health status in SF-36. Symptoms of WMSDs were most reliable risk factor for HRQoL. Various health promotion interventions are needed to prevent WMSDs in all farmers and especially vinylhouse workers.