The member deflection is one of the most important considerations for the serviceability evaluation of reinforced concrete (RC) structures, and the concept of the effective moment of inertia has been generally used for its estimation. However, the actual service load applied on an existing RC beam may not be easily obtained, for which the estimation of beam deflection by existing methods can be difficult to obtain. Therefore, based on the correlation between cracks and deflection in a RC beam, this study proposed a method to estimate the deflection of RC beams directly from the condition of cracks not using the actual loads acting on the member as its input data. The proposed method extensively utilized the relationships among sums of crack widths, average strains, and curvatures, and modification factors obtained from regression analysis were also introduced to improve its accuracy. The deflections of members were successfully estimated by the proposed method independent from applied loads, which was also easy to apply compared to the existing methods based on the effective moment of inertia.