HL-1 cells are the adult cardiac cell lines available that continuously divide while maintaining an atrial phenotype. Here we examined the expression and localization of inositol 1,4,5-trisphosphate receptor ($IP_3R$) subtypes, and investigated how pattern of $IP_3$-induced subcellular local $Ca^{2+}$ signaling is encoded by multiple $IP_3R$ subtypes in HL-1 cells. The type 1 $IP_3R$ ($IP_3R1$) was expressed in the perinucleus with a diffuse pattern and the type 2 $IP_3R$ ($IP_3R2$) was expressed in the cytosol with a punctate distribution. Extracellular ATP (1 mM) elicited transient intracellular $Ca^{2+}$ releases accompanied by a $Ca^{2+}$ oscillation, which was eliminated by the blocker of $IP_3Rs$, 2-APB, and attenuated by ryanodine. Direct introduction of $IP_3$ into the permeabilized cells induced $Ca^{2+}$ transients with $Ca^{2+}$ oscillations at ${geq}$ 20 ${mu}m$ of $IP_3$, which was removed by the inhibition of $IP_3Rs$ using 2-APB and heparin. $IP_3$-induced local $Ca^{2+}$ transients contained two distinct time courses: a rapid oscillation and a monophasic $Ca^{2+}$ transient. The magnitude of $Ca^{2+}$ oscillation was significantly larger in the cytosol than in the nucleus, while the monophasic $Ca^{2+}$ transient was more pronounced in the nucleus. These results provide evidence for the molecular and functional expression of $IP_3R1$ and $IP_3R2$ in HL-1 cells, and suggest that such distinct local $Ca^{2+}$ signaling may be correlated with the punctate distribution of $IP_3R2s$ in the cytosol and the diffuse localization of $IP_3R1$ in the peri-nucleus.