The purpose of this study is to investigate the appropriate metabolite as biomarker for the biomonitoring of 1,3-butadiene(BD) inhalation exposure. We measured the hemoglobin adducts which were extracted from the blood of the ICR mice inhalation exposure with 100ppm and 500ppm 1,3-butadiene for 2 weeks(5 hr/day ${ imes}$ 5 days/week). Hemoglobin adducts were the (N-2-hydroxy-3 -butenyl) valine (HB Val) and (N-2,3,4-trihydroxy-butyl)valine (THB Val). Body weights of the exposure groups were significantly lower from 11 exposure post-day in 100ppm BD inhalation mice and from 7 exposure post-day in 500ppm BD inhalation mice than in control. The levels of HB Val are 0.8~1.7pmol/mg globin for 100ppm BD inhalation exposure, and 2.1~4.4 pmol/mg globin for 500ppm BD inhalation exposure. The levels of THB Val are 15.0~22.0 pmol/mg globin in 100ppm BD inhalation exposure, and 34.8~45.7 pmol/mg globin for 500ppm BD inhalation exposure. So the levels of THB Val and HB Val are proportional relationship with BD exposure level. THB Val is 12.9~18.8 times higher level that HB Val in 100ppm BD exposure group and 10.4~16.6 times higher level than HB Val in 500ppm BD exposure group. We concluded that THB Val is an appropriate metabolite as biomarker for the biomonitoring for BD inhalation exposure.