Recent studies suggest that prolonged standing at work is associated with varicose veins (VV). The objective of this study was to analyze the differences of VV risk in terms of work types in casino dealers and hotel service workers. Symptoms of VV were evaluated using a self-reported questionnaire from 2,092 participants, and muscle fatigue in the lower legs were evaluated using surface electromyography. The shift rate of median frequency in Gastrocnemius was -30.0% among the casino dealers working in prolonged static standing posture, and - 14.3% among the casino dealers sitting during working time. After adjusting age, gender, smoking, and family history of VV, the risk ratio of VV was 3.67(95% CI 2.03-6.59) in group I (prolonged standing and occasional walking at work), 6.29(95% CI 3.48-11.35) in group II (prolonged standing, occasional walking and heavy lifting at work), and 8.07(95% CI 4.51-14.43) in groupIII(prolonged static standing at work). In conclusion, prolonged standing at work may be a work related risk factor of VV.