Purpose: The purpose of this study was to investigate electromyographic(EMG) activity of vastus medialis oblique(VMO) and vastus lateralis(VL) following the application of patellar taping during stair stepping. Methods: Both VMO-VL onset timing and VMO/VL ratio of 15 participants with patellofemoral pain syndrome (PFPS) were measured using an surface EMG uint(Myosystem 1400A, Noraxon Inc., USA). The measurements were taken under three conditions in random order of patellar taping, placebo taping, and no-taping. Data were analysed using $1{ imes}3$ repeated measures ANOVA. Results: There was a significant difference with patellar taping on VMO/VL amplitude compared with the placebo taping and no-taping conditions during stair stepping, but no significant difference on VMO-VL onset timing. Conclusion: These result indicate that patellar taping on the vastus medialis oblique has effect on the VMO/VL ratio. Increased values of VMO/VL ratio may contribute to patellar realignment and explain the mechanism of pain reduction following patellar taping in participants with PFPS.