The start-up program of service company is able to give the positive effect to the job creation and growth but actually the rate of success for start-up program in service industry low and thus the effective support program is required. Recently, it is required that entrepreneurial spirit for success of start-up program in service industry. We examined entrepreneurial spirit between participation motivation in service industry. The results are as follows. As a result of survey for education program participant, it was found that 4 causes of Entrepreneurial Spirit such as innovation thought, market-oriented thought and art-oriented thought, management thought are all significant and especially, the effect of management thought showed somewhat high level in entrepreneurial spirit. Hereafter, this study may help to guide the motivation of preliminary entrepreneurs as food & beverage, restaurant business, retailer, other service industry.