In this paper, we propose an antenna module with high isolation between Tx and Rx in RFID reader module. The proposed module consists of a quadrafilar antenna and a directional coupler with a switchable dummy load to improve the directivity. The proposed module achieves the isolation higher than 20 dB between Tx and Rx. To show the validity of the proposed scheme, we have performed the measurement of tagging range and multi-tagging ability. The experiment results show that the detecting range and multi-tagging ability are enhanced by 81% and 200%, respectively.