Previous laboratory studies have shown that prosodic structures are encoded in the modulations of phonetic patterns of speech including suprasegmental as well as segmental features. Drawing on a prosodically annotated large-scale speech data from the Buckeye corpus of conversational speech of American English, the current study first evaluated the reliability of prosody annotation by a large number of ordinary listeners and later examined whether and how prosodic prominence influences the phonetic realization of multiple acoustic parameters in everyday conversational speech. The results showed that all the measures of acoustic parameters including pitch, loudness, duration, and spectral balance are increased when heard as prominent. These findings suggest that prosodic prominence enhances the phonetic characteristics of the acoustic parameters. The results also showed that the degree of phonetic enhancement vary depending on the types of the acoustic parameters. With respect to the formant structure, the findings from the present study more consistently support Sonority Expansion Hypothesis than Hyperarticulation Hypothesis, showing that the lexically stressed vowels are hyperarticulated only when hyperarticulation does not interfere with sonority expansion. Taken all into account, the present study showed that prosodic prominence modulates the phonetic realization of the acoustic parameters to the direction of the phonetic strengthening in everyday conversational speech and ordinary listeners are attentive to such phonetic variation associated with prosody in speech perception. However, the present study also showed that in everyday conversational speech there is no single dominant acoustic measure signaling prosodic prominence and listeners must attend to such small acoustic variation or integrate acoustic information from multiple acoustic parameters in prosody perception.