To analyze the cooling performance in the refrigerator truck according to frost growth, the analytical model of refrigeration system was developed under frost and non-frost condition using EES. The system performance was analyzed with outdoor temperature, storage temperature, outdoor front air velocity and compressor speed in order to investigate the system performance characteristics with operating conditions. Besides, the system performance under frost condition was compared with that under non-frost condition. As a result, the frost thickness was 0.9 mm when the refrigerating capacity of frost condition was decreased by 30%. The maximum of the system COP was shown at compressor speed of 1500 rpm for non-frost and frost condition, simultaneously. The performance under frost condition was more sensitive to the operating condition compared to that under non-frost condition.