생물다양성과 생태계를 파괴하는 문제잡초인 가시박을 천연물질인 d-Limonene을 이용하여 방제하고자 시험한 결과 온실에서는 본엽 1.5 엽기 및 3엽기 경엽처리시 처리농도가 높으면 높을수록 살초효과가 현저하였다. 노지에서는 본엽 2엽기, 3엽기, 5엽기까지는 처리농도가 높으면 높을수록 그 효과가 현저하였다. 특히, d-Limonene의 $70mg;mL^{-1}$ 처리시 뚜렷한 방제효과를 보였으나 17.5, $35mg;mL^{-1}$ 저농도에서는 효과가 다소 낮았다. 또한 노지에서의 본엽 15엽기 내외는 d-Limonene의 방제효과가 낮았다. 온실조건에 비하여 노지조건에서 d-Limonene의 처리효과가 우수하였다. 가시박 방제를 위해 d-Limonene 처리시 가시박 5엽기 까지는 $70mg;mL^{-1}$을 처리하는 것이 방제효과가 가장 현저할 것으로 판단되었다.
This experiment was conducted to Investigate the herbicidal activity of naturally developed d-Limonene on the control of Sicyos angulatus L. which is destructive to biodiversity and ecosystem under greenhouse and open field condition. Among the tested concentrations, most efficacy of early foliar application(leaf stage with 1.5 and 3) of d-Limonene was found in $70mg;mL^{-1}$, followed by $35mg;mL^{-1}$, $17.5mg;mL^{-1}$ in that order under greenhouse condition. The higher the d-Limonene concentration, the better the effect in greenhouse condition. In leaf stage with 2, 3 and 5 under open field condition, the higher the d-Limonene concentrations, the higher the weeding effect. The remarkable effect was, particularly, found in d-Limonene with $70mg;mL^{-1}$ while little was found in the plants with d-Limonene with $35mg;mL^{-1}$. In the case of 15 leaf stage of S. angulatus L. treated with d-Limonene under outside treatment effect was low. The treatment effect of d-Limonene under open field compared with greenhouse condition was more significant. It can be recommended that d-Limonene with $70mg;mL^{-1}$ could control S. angulatus L. by 5 leaf stage.
