The heat transfer and pressure loss characteristics of a rotating two-pass channel with a guide vane in the turning region have been studied using three-dimensional Reynolds-averaged Navier-Stokes (RANS) analysis, and the shape of the guide vane has been optimized using surrogate modeling optimization technique. For the optimization, thickness, location and angle of the guide vanes have been selected as design variables. The objective function has been defined as a linear combination of the heat transfer and the friction loss related terms with a weighting factor. Latin hypercube sampling has been applied to determine the design points as design of experiments. A weighted-average surrogate model, PBA has been used as the surrogate model. The guide vane in the turning region does not influence the heat transfer in the first passage upstream of the turning region, but enhances largely the heat transfer in the turning region and the second passage. In an example of the optimization, the objective function has been increased by 13.6%.