Relay-assisted multiple input multiple output (MIMO) technique has become a promising candidate for next generation broadband wireless communications. In this paper, we propose maximum ratio combining (MRC) minimum mean-square-error (MMSE) equalization for single carrier-frequency domain equalizer (SC-FDE) in amplify-and-forward (AF) relaying networks. The performance of SC-FDE system can be improved considerably by achieving both the diversity gain and the MMSE equalization gain when the signals from source-destination (S->D) and source-relay-destination (S->R->D) are combined and equalized by means of the MMSE criteria. We find the weighting coefficients of MRC combining and the tap coefficients of MMSE equalizer for SC-FDE in AF relaying networks. Simulation results show that the proposed relay-based system considerably outperforms the conventional SC-FDE system.