Objectives: The objectives of this study were to describe population-based prevention strategies for childhood obesity and to discuss its application in a city in Korea. Methods: Literature review and empirical findings for ongoing programs were performed to develop population-based prevention strategies for childhood obesity with the framework and principles of WHO population-based prevention strategies for childhood obesity. Results: The developed framework had five key strategies (supportive policies, supportive environment, supportive program, strategic development & leadership, and monitoring & evaluation) under hierarchic objectives (long-term, middle-term and short-term) with the vision of healthy growth and development of all children and youth. Each strategy included evidence-based action plans with WHO principles. Conclusions: The developed strategies have advanced the existing strategies for childhood obesity prevention by providing the sustainable and systematic framework and action plans based on ecological approach. Further, the feasibility for operating the strategies needs to be verified.