Objectives: This study aims to evaluate the outcomes of an education program for public health service workers in public hospitals, utilizing the Kirkpatrick model. Methods: The study participants were 118 staff in 48 public hospitals. Of the stages in the Kirkpatrick model(reaction, learning, behavior, and result), reaction and learning stages were analyzed in this study. A 10-item self-evaluation questionnaire was used to measure satisfaction level for the reaction, and achievement of learning purposes for the learning. The education program consisted of general courses and special two tract courses(Tract A: chronic diseases, Tract B: health promotion). Results: The highest score for reaction was for Tract A(score=4.4), whilst the lowest score for reaction was for lecture(score=3.0). Learning achievement was significantly different between pre-education and post-education(p<0.01), except for health technicians. Conclusions: The results of this study could be utilized to develop effective systematic education programs for public health service workers in public hospitals.