Purpose: We investigated the effects of physical practice, mental practice, and cross education using serial reaction time (SRT) in the non-dominant left arm of right-handed individuals. Methods: Subjects were divided into three groups; physical practice (n=8), mental practice (n=8) and controls (n=8). They did, respectively, physical training or mental training, or had no intervention for three weeks. Super lab 4.0 displayed four symbols on the monitor and subjects were asked to push the matching button. Reaction time was assessed pre- and post-intervention. Results: Reaction time was significantly lower after physical practice (p<0.05), but mental practice did not significantly lower reaction time? in the left hand. Also, reaction time was not significantly lower after physical practice or mental practice in the right hand. Conclusion: Physical practice can improve motor learning, but mental practice is not sufficient. Also, neither physical practice nor mental practice is sufficient for cross education from the nondominant to the dominant arm.