This study was conducted to investigate runoff characteristics of non-point pollutants source at the urban and rural zones in sangju area. The monitoring was conducted with seven events for ten months and Event mean Concentration(EMC) and First Flush Effect(FFE) of SS and BOD were calculated on the result of the water quality parameters. During rainfall event, the peak concentrations of SS and BOD were observed after 3~4 hours of rainfall in rural areas. Whereas, the peak concentrations occurred within 1~2 hours after rainfall and then the highest concentration of NPS pollutants sharply decreased, showing strong first flush effect in urban areas. The cumulative load curves for NPS pollutants showed above the $45^{circ}$ straight line, indicating that fist flush effect occurred in urban areas. The mean SS EMC values of rural areas ranged from 0.9~3.3mg/L, it was higher value when compare to urban areas. While the mean BOD values of urban areas were shown the highest values.