We have performed optical design of light guide plates (LGPs) for a photobioreactor by using an illumination design tool. An LGP having light emitting diodes (LEDs) as a light source on the four sides was designed by optimizing the distribution of LGP patterns and design of another LGP, on top of which sunlight was focused by a Fresnel lens, was executed. Finally, a hybrid-solar-and-LED-lighting scheme was described. Detailed design process and optical performances of the designed LGPs are presented.