This paper proposes an efficient code acquisition scheme for direct-sequence spread spectrum (DSSS) based real-time locating systems (RTLS). The proposed scheme employs multiple bit integration to significantly decrease false alarms when no packet signal is transmitted and increase the detection probability when the RTLS tags are excited. The performance of the proposed code acquisition scheme is analyzed and numerically evaluated, and compared with those of single-dwell and traditional double-dwell code acquisition schemes. The simulation results show that the proposed multiple-bit integration scheme outperforms single-dwell and double-dwell schemes.