In this paper, we suggest the object-oriented methodology for the design and implementation scheme for the program development in the application of control and instrumentation such as the detection system of RFID defective tags which needs the embedded programming. We apply the design methodology of UML in the system design phase, and suggest the implementation scheme of LabVIEW programs using LVOOP(LabVIEW Object Oriented Programming)in which make it possible to write the object-oriented programming. We design the class diagram and the sequence diagram using UML, and write the classes of LVOOP from the designed class diagram and the main VI from the sequence diagram, respectively. We show that it is possible to develop the embedded programs such as the RFID application through the implementation example of the detection system of RFID defective tags in this paper. And, we obtain the advantages based on the object-oriented design and implementation using the LVOOP approach such as the development of LabVIEW programs by adding the classes and the concept of object of the object-oriented language to LabVIEW.