In order to acquire new information and biological meaning of the signal data by defining the relationships between them, new modeling technique, ontology, has been proposed. The data of bio-signal can be represented as a systematic and logical to manage continuously bio-signal data using ontology. Furthermore, knowledge of which resources are utilized to provide improved service quality in medical information, health services in various fields. However, relevant studies have not been performed actively to compare importance of relationships between bio-signals. Therefore semantic representation of biometric information should be by defining the relationship between bio-signals. In this paper, we have developed bio-signal ontology to use as a model for using domain knowledge. We verified the usefulness of the ontology by using scenarios.