More recently, variety RFID (Radio Frequency Identification) readers are produced by RFID equipment manufactures. Although a transmission standard instituted by EPCglobal is proposed for data transmission between the RFID readers and tags, other RFID reader protocols and the communication connection methods are be in use in other RFID companies. To replace or add the RFID readers of an RFID system, the developers should make changes to the core of the application and/or middleware. In this paper, we propose an RFID reader framework which can manage RFID readers without having to make changes the code of the application in environment with the growing number of heterogeneous RFID readers.This framework that sits on the layer between the RFID readers and the applications provides transparency to the RFID readers. Additionally, it can be used for monitoring the state and the property of all connected RFID, and for recording the RFID tag event logs and system logs. By using this framework, heterogeneous readers can be replaced and added without writing additional code in the applications. Consequently the readers can be easily managed and controlled by the RFID system administrator.