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Rapid and Simple Biochemical Detection for Salmonella spp. Using Modified LB Broth and the MUCAP Test
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  • Rapid and Simple Biochemical Detection for Salmonella spp. Using Modified LB Broth and the MUCAP Test
  • Rapid and Simple Biochemical Detection for Salmonella spp. Using Modified LB Broth and the MUCAP Test
저자명
Jang. Keum-Il,Ahn. Jun-Bae,Kim. Kwang-Yup
간행물명
Food science and biotechnology
권/호정보
2011년|20권 1호|pp.201-207 (7 pages)
발행정보
한국식품과학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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In this study, we developed a rapid Salmonella detection system and confirmed its detection efficiency using modified selective pre-enrichment and the 4-methyl umbelliferyl caprilate (MUCAP) test. Luria-Bertani (LB) medium without auto-fluorescence was modified by the addition of a bile salt mixture and sodium citrate as inhibitors, after which ferrous sulfate and sodium thiosulfate were added for the induction of $H_2S$ gas. In a mixed culture of Staphylococcus aureus, Escherichia coli, and Salmonella enteritidis in Salmonella detective modified LB (SDMLB) medium, S. enteritidis dominated regardless of their initial population size. Among 10 bacterial strains, Salmonella and Enterobacter spp. were cultured and distinguished by the $H_2S$ test because the Enterobacter spp. was unable to produce $H_2S$ gas in SDMLB medium. MUCAP testing produced blue fluorescence only for Salmonella spp. Tests for Salmonella using chicken skin and egg shells revealed the presence of Salmonella spp. Thus, foods carrying Salmonellae were detected rapidly and simply using this method.