The paper deals with the vibration isolation of a large structure using an experimental technology. In the case of vibration isolation for the vicinity of a subway or a railroad station, most of vibration isolation techniques using isolation materials with high isolation efficiency only, have been applied. Therefore, the quantitative evaluation and design technologies are required for a vibration isolation of large structures. In this study, firstly, vibration characteristics due to train or subway are analyzed. Secondly, the performance of existing vibration isolation materials such as precision isolation material, elastomer is estimated through the experiments. Thirdly, the performance of a tire isolation material and its frame is tested and evaluated. Finally, it is shown that tire isolation materials can be applied to the vibration isolation or vibration reduction of large structures.