Dyeing properties of cotton and ramie fabrics with pine needles colorants were studied by investigating the analysis of pine needles colorants, the effect of dyeing conditions (such as dye concentration, dyeing temperatures and times on dye uptakes), effect of mordants, and color change. The various colorfastness of dyed fabrics were evaluated for practical use; in addition, the antimicrobial ability, ultraviolet-cut ability, and deodorant ability were estimated. In the UV-Visible spectrum, the wavelength of the maximum absorption for pine needles extract was 285nm, and pine needles colorants produced a yellow color. From FT-IR and GC-MS results, it was assumed that chromophoric substance from pine needles extracts were mixed with flavonol tannin and flavanol tannin. An increased dyeing concentration resulted in a larger dye uptake and a Freundlich absorption isotherm was obtained. A larger dye uptake occurred as the dyeing time and temperature increased. Post-mordanting was more effective than pre-mordanting and the dye uptake of fabrics improved by mordanting. The colorfastness of dyed fabrics showed a low rating; however, colorfastness to washing and dry cleaning of cotton fabrics mordanted with N.Cu, and friction fastness of ramie fabric mordanted with Cu improved. The dyed fabrics showed very good antimicrobial abilities of 99.9%. In addition, the ultraviolet-cut ability and deodorant ability improved in fabrics dyed with pine needles extracts.