Purpose: This study was to compare differences between both eyes in corneal powers, axial lengths, anterior chamber depths in anisometropia and isometropia, and to investigate the relationship between anisometropia and refractive components. Methods: The subject was a total of 83 patients, anisometropia 45 patients (90 eyes) and isometropia 38 patients (76 eyes) from 2.7 to 15.3 years old, prescribed eyeglasses and contact lenses by refraction from July 2010 to August 2010 in Gwangju City B eye clinic. Axial length, anterior chamber depth, corneal curvature, and corneal refractive power were measured using IOL Master. Refractive error was measured using an Auto-refractometer. Results: Anisometropia was a statistically significant difference in axial length, binocular refractive components, refractive error, and axial length, Axial length/corneal radius (AL/CR) ratio showed a statistically significant difference in anisometropia and isometropia. The major cause of anisometropia all 45 subjects was the axial length. Among the refractive components axial length, AL/CR had a strong correlation, but corneal refractive power had no correlation. Anterior chamber depth had a weak correlation. Conclusions: This study found that refractive error was the most axial ametropia caused by the axial length. The main cause of anisometropia was the axial length, but refractive components had a weak correlation.