In this study, we identified wood species used in manufacturing Streetcar No. 381 from Seoul Museum of History. This Streetcar No. 381 is an important cultural heritage as one of the 2 streetcars remained in Seoul. It is important to preserve this streetcar through restoration and to maintain without any damage or degradation. The information on species of wood members should be needed for its original restoration. Thus, 6 interior and 1 exterior wood members were sampled and identified microscopically. Among the 7 wood members identified, 2 were found to be douglas-fir ($Pseudotsuga$), 2 lauan ($Shorea$), 1 exotic hard pine of ponderosa group ($Pinus$), 1 larch ($Larix$), and 1 oak of cerris group ($Quercus$), respectively.