We describe, throughout a Mach-Zehnder interferometric configuration, a new test sieves calibration method based on off-axis digital holography. The experiment is conducted on a test sieve of square openings. The nominal sieve opening is 1.00 mm with maximum individual opening of 1.14 mm in size. The recorded off-axis hologram is numerically processed using Fresnel transforms to obtain an object wave (amplitude and phase). From the reconstructed phase, the average size of the illuminated openings has been measured precisely. The proposed method can provide a real time solution for calibrating test sieves very precisely and with moderate accuracy.