This paper presents the transient performance analysis of a micro-hydro Pelton turbine for the osmotic power generation using the commercially available computational fluid dynamics (CFD) code, ANSYS CFX. The detailed flow field in the micro Pelton turbine with a single-jet is investigated by the CFD code adopted in the present study. Predicted characteristic curves agree fairly well with measured data for a prototype Pelton turbine over the normal operating conditions. The computational analysis method presented herein can be effectively applied to the hydraulic design optimization process of general purpose Pelton turbine runners.