Purpose: The purpose of this study was to examine the relationship between professional nursing values and career preparation behaviors in senior nursing students. Method: The sample consisted of 199 nursing students before getting jobs who attended a nursing vocational college in Daegu and a nursing department of a university in J city of Chungcheongbuk-do. The data were collected from the first of May to the first of June in 2010. Results: The mean score was 106.76 for professional nursing values. The highest reported score of the nursing professional values domains was professionalism of nursing and was followed by nursing practical role and professional self-concept. The mean score was 42.66 for career preparation behavior. There was alow correlation between professional nursing values and career preparation behaviors. Conclusion: This finding suggests nursing students have differences from career preparation behaviors according to viewpoints about nursing as a professional job. Therefore, it is necessary to develop new curriculum and reinforce existing education for developing of professional nursing values. Moreover, nursing education institutes should set up educational objectives related to their general characteristics for improving career preparation behavior.