The present study was carried out to evaluate the antioxidant effect of methanol extract of $Pandanus$ $odoratissimus$ lim (MEPO) leaf in wistar albino rats. The animals were grouped differently and administered with Carbon tetrachloride ($CCl_4$) (1.5/$kg^{-1}$ $CCl_4$ 1 ml $kg^{-1}$ in liquid paraffin 3 doses (i.p) at 72 h interval). The extracts at the doses of 50 and 100 mg $kg^{-1}$ and Liv 52 25 mg $kg^{-1}$ were administered to the $CCl_4$ treated rats. The effect of extract and Liv 52 on serum transaminase (GOT, GPT), alkaline phosphatease (ALP), bilirubin and total protein were measured in the rat induced by $CCl_4$. The effect of extract on lipid peroxidation (LPO), Superoxide Dismutase (SOD), Catalase (CAT), Glutathione (GSH) and Vitamin E were estimated. The extract and Liv 52 produced significant ($P$ <0.05) effect by decreasing the activity of serum enzymes, bilirubin, uric acid and lipid peroxidation and significantly ($P$ <0.05) increased in the level of SOD, CAT, GSH, Vitamin E and protein. Hence these results suggest that Methanolic extract of Pandanus odoratissimus has potent antioxidant properties.