Nonylphenol (NP) is considered an endocrine disruptor due to its weak ability to mimic estrogen and in turn disrupt the natural balance of hormones in affected organisms. NP is reported to cause negative health effects in humans, such as hormone abnormalities and inhibition of growth and reproduction. In the present study, we developed a molecular tool for the evaluation of endocrine toxicity in mouse. To identify gene regulation effects of NP, we estimated gene expression in mouse Sertoli (TM4) and germ cell (GC) lines after exposure to NP. We measured the $IC_{30}$ value of NP, then exposed the cells to that concentration for 3 hr and 24 hr and used EndoTox Array. The EndoTox Array was manufactured to monitor the endotoxicity of environmental chemicals. This array contains 1306 genes that are influenced in reproductive toxicity or by EDCs. In the expression pattern analysis, 28 genes related to the reproductive process, cell proliferation, and nervous system development were progressively changed over time in NP-exposed cells. The study of gene interaction will increase our understanding of the time-dependent molecular mechanisms of NP.