An actinomycete strain, designated $M1T8B9^T$, was isolated from cow dung in Suwon, Republic of Korea. The isolate was a Gram-positive, nonmotile, and non-spore-forming bacterium. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the genus Microbacterium, with its closest neighbors being Microbacterium soli $DCY17^T$ (98.2%) and Microbacterium esteraromaticum DSM $8609^T$ (98.0%). The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, and one unknown glycolipid. Strain $M1T8B9^T$ contained the major fatty acids $C_{15:0}$ anteiso, $C_{16:0}$ iso, $C_{17:0}$ anteiso, and $C_{15:0}$ iso, and the cell-wall peptidoglycan was of type $B2{eta}$. According to DNA-DNA hybridization studies, strain $M1T8B9^T$ showed 42% and 26% relatedness with M. soli $DCY17^T$ and M. esteraromaticum DSM $8609^T$, respectively. On the basis of the data presented, strain $M1T8B9^T$ is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium suwonense sp. nov. is proposed. The type strain is $M1T8B9^T$ (=KACC $14058^T$ =NBRC $106310^T$).