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Separation of Taxanes from Taxus canadensis Using Dynamic Pressurized Liquid Extraction
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  • Separation of Taxanes from Taxus canadensis Using Dynamic Pressurized Liquid Extraction
  • Separation of Taxanes from Taxus canadensis Using Dynamic Pressurized Liquid Extraction
저자명
Wang. Yuheng,Gamage. Joanne,Zhang. Zisheng
간행물명
Biotechnology and bioprocess engineering
권/호정보
2011년|16권 4호|pp.769-776 (8 pages)
발행정보
한국생물공학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

An extraction technique, dynamic pressurized liquid extraction (DPLE), was proposed to extract the taxanes; including 10-DAB III, Baccatin III, 9-DHB III and paclitaxel, from powdered Taxus canadensis needles. A dual-solvent approach was adopted in which the impurities were firstly removed by extraction with hexane, and the taxanes were subsequently extracted with an appropriate solvent. The performance of chloroform, dichloromethane, and mixtures of methanol/dichloromethane was compared for use as the taxane-extracting solvent, and it was found that solvents containing a higher proportion of methanol had higher extraction capabilities. The effect of temperature on DPLE extraction of the taxanes was also studied, and it was found that higher extraction efficiencies could be realized with increasing temperature up to a threshold of $90^{circ}C$. Based on a progressive conversion model, a kinetic equation for the extraction process was proposed. This model successfully confirmed that smaller needle powder particle sizes would result in higher extraction rates, which is consistent with the data obtained by experimentation.