Nano-$TiO_2$ based multilayer nanocomposite films were fabricated on cationically modified woven cotton fabrics by layer-by-layer molecular self-assembly technique. Cationization process was used to obtain cationic surface charge on cotton fabrics. Attenuated total reflectance Fourier transform infrared spectroscopy analyses were used to verify the presence of cationic surface charge and multilayer films deposited on the fabrics. Scanning electron microscope micrographs of poly(sodium 4-styrene sulfonate)/$TiO_2$, nano polyurethane/$TiO_2$, and $TiO_2$/poly(diallyldimethylammonium chloride) multilayer films deposited on cotton fabrics were taken. With nano-$TiO_2$ based multilayer film deposition, the protection of cotton fabrics against UV radiation is enhanced. The UV protection durability of the self-assembled multilayer films deposited on the cotton fabrics was analyzed after 10 and 20 washing cycles at $40^{circ}C$ for 30 min. Air permeability and whiteness value analysis were performed on the untreated and multilayer film deposited cotton fabrics. The effect of layer-by-layer deposition process on tensile strength properties of the warp and weft yarns was determined.