A diblock rod-coil aromatic amphiphilic molecule, consisting of three biphenyls and one styrene unit linked together with ether bonds as a rigid rod segment and poly(ethylene oxide) with the number of repeating units of 34 as coil segment, has been synthesized. The self-assembling behavior of the molecule is investigated by means of differential scanning calorimetry (DSC), small-angle X-ray scattering (SAXS), dynamic light scattering (DLS) and scanning electron microscope (SEM) in the solid state or an aqueous solution. This molecule spontaneously self-assembles into lamellar structure in the crystalline phase. In aqueous medium, the molecule self-organizes into spherical micelles and the micelles can be used as nanoreactor for Suzuki coupling reaction at room temperature.