Cuttlefish (Todarodes pacificus) viscera were tried to extract bioactive oil containing high amount of ${omega}$-3 fatty acids. Cuttlefish oil (CFO) was extracted from the viscera, refined, and evaluated. The tried effective extracting condition was 9 h hot steam extraction. Raw CFO contained high ${omega}$-3 fatty acids (FA); 21.4% docosahexaenoic acid (DHA), 11.3% eicosapentaenoic acid (EPA). The raw CFO was refined by acetone fractionation at the optimum conditions ($-30^{circ}C$, 8 h). One advantage of the fractionated CFO was high amounts of DHA (28.6%) and EPA (14.7%). Also, another advantage of the refined CFO was its stability during 92-day storage period at $50^{circ}C$. This was seen by measuring the acid values (AV<1.0) and peroxide values (POV<2.1) throughout the storage period. In conclusion, the refined CFO containing ${omega}$-3 FA (>43%) has potential as a good functional ingredient.