Ubiquinone-10 (CoQ10), a vitamin-like lipophilic component of the membrane-bound electron transport system, has a wide range of therapeutic, neutraceutical, and cosmeceutical applications. The objective of this study was to optimize nutritional requirements for production of CoQ10 by Paracoccus dinitrificans NRRL B-3785 fermentation. Effect of type and concentration of carbon and nitrogen source on fermentation kinetic parameters were analyzed using logistic and Luedeking-Piret equations. In submerged batch fermentation, yield of CoQ10 was 12.22 mg/L when 40 g/L glycerol was used and specific growth rate (0.056/h) as well as growth associated constant (${alpha}$=0.680 mg/g) were higher as compared to other concentrations. Ammonium nitrate and proteose peptone at 5 (${alpha}$=0.677 mg/g; ${eta}$=0.0072 $mg/g{cdot}h$) and 20 g/L (${alpha}$=0.806 mg/g; ${eta}$=0.0074 $mg/g{cdot}h$), respectively, were optimal for CoQ10 production. CoQ10 formation observed to be both growth and non-growth associated. In optimized medium CoQ10 formation increased considerably from 1.91 to 14.12 mg/L.